Quantitative model for gene regulation by lambda phage repressor.

Regulation by N gene protein of phage lambda of anthranilate synthetase synthesis in vitro.

The N protein of bacteriophage lambda is a positive regulator of early lambda gene expression. In a lambdatrp transducing phage, lambdatrp46Nam, the synthesis of trp enzymes in vivo is also dependent on the presence of active N protein. The DNA of this phage has been used in a protein-synthesizing system in vitro to develop a biochemical assay for the activity of the N protein. From the followi...

Down Regulation of DHFR Promoter Activity by PML Transcription Repressor

Control of transcription of the repressor gene in bacteriophage lambda.

The rate of transcription of the structural gene for repressor (cI gene) in bacteriophage lambda is controlled by the amount of active repressor in the cell. When the (reversibly) thermolabile repressor in a bacterium lysogenic for lambdacI(857) is inactivated by heat, the rate of repressor gene transcription immediately falls. If the repressor is renatured, synthesis of repressor messenger is ...

constructing a dna ladder range for lambda phage by multiplex pcr

background and objectives: dna ladder contains dna fragments of different length but with known size, used to determine the size of unknown dna molecules. different dna ladders are available for expected dna length. conserved sequences were selected for design of primers to generate dna fragments of known specific size. materials and methods: in this study, we describe a method by which dna lad...

down regulation of dhfr promoter activity by pml transcription repressor

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